Dog normal serum (unspecified gender) (10 ml)
Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.
Goat anti-Rabbit IgG (unabsorbed)
Antibody purity is > 95% based on SDS-PAGE.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 0.05% (w/v) with sodium Azide is added as a preservative.Based on IEP, this a
AgriseraECL Bright
User Instruction Store reagents A and B in the darkness at 4-8°C.Mix equal volumes of reagent A and B (chemiluminescent substrate) in a clean container and equlibrate to room temperature 30 minutes b
AgriseraTMB HRP Substrate
One component ready to use reagent. It contains 2.5 mMol L-1 TMB and Hydrogen Peroxide in a buffer at pH 3.6. It also contains non-toxic stabilizers.